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Team reports first direct evidence that transplanted muscle cells can take root in damaged heart

The first direct evidence that muscle cells transplanted from within a heart patient's body could help heal damaged heart muscle is being reported by a team from the U-M Health System (UMHS), Massachusetts General Hospital and Diacrin Inc.

The results come from three patients who had cells from their thigh muscles injected into their heart muscle while awaiting heart transplants, then allowed their old, damaged hearts to be examined for signs of cell growth after getting new hearts.

The detailed examinations, the first of their kind, showed that the injected cells not only survived in the new environment, but began to form muscle fibers. The areas where cells were injected also had an increase in the formation of small blood vessels. None of the patients experienced immune reactions to the cell transplants.

"These results give us the first indication that muscle cell transplants, even from an entirely different kind of muscle, could one day be used to help repair damaged, failing hearts without danger of rejection," says U-M cardiac surgeon Dr. Francis Pagani, who presented the results at the Scientific Sessions of the American Heart Association. "We have much further to go, but we're very encouraged."

The detailed findings come from one arm of a two-part Phase I study sponsored by Diacrin, designed to see if transplanted skeletal muscle cells might be a feasible option for repairing hearts damaged by heart attack and other diseases.

The study relies on the premise that certain kinds of cells can be expanded in culture and maintain their functional characteristics. Though similar to the concept of "stem cells" that can become any kind of cell in the body, the study uses "satellite cells," which occur naturally in muscle and help repair damage by dividing and moving to injured areas.

The arm of the study conducted at UMHS and Temple University involves patients awaiting heart transplants to replace their scarred, failing hearts, and scheduled to receive implanted heart-assisting devices called left ventricular assist devices (LVADs) to help them survive until a new heart became available. Because the old heart can be removed for tests after the transplant, detailed analysis of the injected cells is possible.

The other arm of the study, led by the Arizona Heart Institute, looked at how well patients tolerate different doses of transplanted skeletal muscle cells injected during heart bypass surgery. It assessed the safety of the cell injection and found indirect evidence of scar tissue regeneration, but it could not examine the hearts directly.

In both arms of the study, a sample of cells was removed from the quadriceps muscle, and treated with enzymes to isolate the satellite cells. They were then grown in a laboratory under carefully controlled conditions to give the original handful of cells time to divide and produce 300 million cells.

Then, surgeons injected the cells into the wall of the heart's pumping chamber during an open-heart surgical procedureeither the LVAD implantation surgery or a coronary artery bypass graft (CABG) operation.

In LVAD patients, the new cells were placed in cardiac muscle tissue that had been severely scarred and hardened to the point that it could no longer contract sufficiently to help pump blood. The LVAD helps boost the patient's pumping power by feeding blood into a battery-powered metal pump and out through a tube connected to the main artery. UMHS teams have implanted more than 100 patients with a type of LVAD called a Heartmate in the past six years.

The new results come from an analysis of two UMHS patients and one Temple patient. Two other U-M LVAD patients have received cell injections but still are awaiting heart transplants.

"The results show direct evidence of skeletal muscle cell survival and differentiation into mature muscle fibers, measured using antibodies that specifically target skeletal muscle cells," Pagani says. "Because cardiac muscle and skeletal muscle are two distinct types of tissue, the antibody test shows conclusively that the transplanted skeletal muscle satellite cells survived."

The transplanted cells also appear to have begun forming vascular muscle cells, which make up the walls of blood vessels.

In addition to the encouraging finding that the injected cells "grafted" into their new environment, the results show on a molecular level that the heart muscle did not reject the newcomers.

"Because the skeletal muscle cells are from the patient's own body, we don't expect the kind of immune reaction and rejection that we often see in transplants of whole hearts from donors," says Pagani, an associate professor of surgery at the Medical School and head of the Heart Transplant Program at the Cardiovascular Center. "If further study bears this finding out, we may have a new option for repairing hearts without putting the patient at risk of dying from rejection, or needing lifelong anti-rejection medications."

Pagani stresses that these early results are encouraging but are merely the first steps in evaluating skeletal muscle cell transplants. Combined with the results from the safety wing of the study, the initial results may help the researchers determine how to proceed toward evaluations of whether cell transplants can help heal patients' hearts.

In addition to Pagani, the research team includes U-M cardiologist and transplant program medical director Dr. Keith Aaronson and research coordinator Sue Wright, a registered nurse, as well as several researchers from Mass General.


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