Insights into honeybee sex gene
could bring sweet success in breeding

What makes a bee a he or a she?

Three years ago scientists pinpointed a gene called csd that determines gender in honeybees, and now a research team led by U-M evolutionary biologist Jianzhi "George" Zhang has unraveled details of how the gene evolved. The new insights could prove useful in designing strategies for breeding honey bees, which are major pollinators of economically important crops—and notoriously tricky to breed.

The findings of Zhang and collaborators appear in a special issue of Genome Research devoted to the biology of the honeybee. The issue was published online and in print Oct. 26, coinciding with the publication of the honeybee genome sequence in the journal Nature.

Scientists long have known that in bees—as well as wasps, ants, ticks, mites and some 20 percent of all animals—unfertilized eggs develop into males, while females typically result from fertilized eggs. But that's not the whole story, and the discovery in 2003 of csd (the complementary sex determination gene) helped fill in the blanks. The gene has many versions, or alleles. Males inherit a single copy of the gene; bees that inherit two copies, each a different version, become female. Bees that have the misfortune of inheriting two identical copies of csd develop into sterile males but quickly are eaten at the larval stage by female worker bees.

The system works fine in nature, where it prevents the colony from wasting precious energy and resources on abnormal males incapable of carrying out the all-important role of mating. But in bees raised for honey or for pollinating crops, the sex-determination system can cause problems. Beekeepers inbreed bees to select desirable traits, but inbreeding raises the odds of producing fertilized eggs with two copies of the same csd allele. If too many sterile males result, the colony may die out.

"If we know more details about how many alleles there are and what their frequencies are, bee breeders can design better strategies to avoid producing sterile males," Zhang says. "Our work aids in this effort by providing a direct tool to examine alleles from different populations."

In the research, Zhang and co-workers from U-M, Michigan State University (MSU) and the University of Kansas sequenced csd genes from individuals in three closely related species of honeybee: the familiar backyard denizen Apis mellifera and the Asian honeybees Apis dorsata and Apis cerana. The group also sequenced six so-called neutral regions of the genome, which, unlike genes, do not carry codes telling cells how to make proteins. Then, the researchers constructed gene genealogies—family trees for both the csd gene and the neutral regions.

"Their results showed that csd is about seven times more variable than neutral regions of the honeybee genome. In addition, many csd variants are shared among the three species, evidence that the many different alleles have been preserved in these lineages for a very long time.

"Such a pattern supports the idea that an evolutionary mechanism known as balancing selection has been at work. Evolution works through the process of natural selection, in which genetic mutations that offer some advantage are favored, and those that have harmful effects are weeded out. Typically, this results in one version of a gene becoming very common and other versions becoming rare or disappearing altogether. When balancing selection operates, however, natural selection favors a diverse mix of alleles, as seen with csd in honeybees.

The research also showed just how long the csd alleles have been around.

"We estimated the age of the alleles at about 14 million years," Zhang says. "We don't know for sure when the species formed, but it's thought to be about 6 to 8 million years ago, so the alleles are even older than the species."

Zhang collaborated on the research with postdoctoral fellow Soochin Cho and undergraduate student Daniel Green of U-M, Zachary Huang of MSU and Deborah Smith of the University of Kansas. The researchers received financial support from the U-M Office of the Vice President for Research, the National Institutes of Health and the University of Kansas General Research Fund.

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